Fig. 6

SMG6 knockdown leads to increased vRNA levels, but not reactivation in J-Lat cells. J-Lat 10.6 cells were either transfected either siNS or siSMG6 and were either uninduced (DMSO) or reactivated (PMA). a Reactivation monitored by GFP production was measured by flow cytometry. Error bars represent the standard deviation from three independent experiments with at least 10,000 cells counted per treatment. (Two-way ANOVA; p > 0.05). b Cell lysates were run on acrylamide gels and SMG6 and pr55^Gag protein levels were detected by Western Blotting. c Example dot plot depicting vRNA expression in siNS PMA and siSMG6 PMA conditions using FISH-Flow technique and, d The % of vRNA expressing cells were quantified. Error bars represent the standard deviation from three independent experiments. Asterisks represent statistically significant difference between groups (One-way ANOVA; p < 0.001)