Fig. 1

Phosphoproteomics of CCR5-tropic HIV signaling in primary memory CD4+ T cells. a Schematic of experimental design. Purified, unstimulated memory CD4+ T cells were exposed to extracellular vesicles (EVs), HIV, or HIV in the presence of 100 μm maraviroc (MVC) for 1, 15, or 60 min. A fraction of the experimental samples were pooled and run in triplicate for technical replicates. b Entry of HIV-1 THRO into primary CD4+ T cells is inhibited by treatment with 100 μM maraviroc. c Peptides demonstrating coefficients of variation (CVs) of > 50% in the technical replicate samples were excluded from further analysis. d Table of summary counts from the phosphoproteomics experiment; FDR-filtered results are selected if meeting the FDR = 0.001 cutoff. e Phosphopeptide-level MA plots across different treatment conditions and time points. Fold change (FC) calculations were in reference to extracellular vesicle control. Mean peptide intensity was calculated between the control and designated treatment condition. Grey circles indicate peptides that do not meet the log2(FC) cutoff