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Fig. 5 | Retrovirology

Fig. 5

From: HIV latency reversing agents act through Tat post translational modifications

Fig. 5

Mutations in Tat at lysine and arginine residues reduce the efficiency of HIV transcription. a Schematic diagram showing Tat protein sub-domains and residues that are post-translationally modified. Specific basic lysine (K) and arginine (R) residues were mutated to alanine (A) in pTat101 (AD8)-Flag using site-directed mutagenesis. b Western Blot analysis of wild-type (WT) or mutant Tat expression in HEK293T cells 48 h post-transfection using anti-Flag antibody. GAPDH was used as a loading control. c Luciferase expression of TZMbl cells 48 h following transfection of Tat mutants compared to WT Tat101, represented as the  % of WT activity. Only statistically significant comparisons are shown ****p < 0.0001 (Paired T test). The black lines represent the mean ± SEM (n = 5). For the letters in coloured circles: P phosphorylation, Ac acetylation, Me methylation, Ub ubiquitination

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