Fig. 2

JQ1 but not HDACi can increase splicing in the absence and presence of Tat. HEK293T cells were co-transfected with the pLTR.gp140/EGFP.Rev∆38/DsRed splicing reporter together with a plasmid expressing Rev, in the absence (a, b) or presence (c, d) of 100 ng of pTat101 (AD8)-Flag expression plasmid and then treated with a panel of LRAs or DMSO diluent control (n = 5). EGFP (unspliced) and DsRed (spliced) expression were measured using flow cytometry. Comparisons of each condition to DMSO were made using 2-way ANOVA test. Only statistically significant comparisons are shown *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The black lines represent the mean ± SEM. DMSO (1:5000), VOR = vorinostat (0.5 μM), PAN = panobinostat (30 nM), JQ1 (+) (1 μM), CTN = chaetocin (30 nM), DIS = disulfiram (500 nM), or PMA/PHA = phorbol myristate acetate/phytohaemagglutinin (10 nM PMA, 10 μg/mL PHA)