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Table 1 Viral clones and stocks used in this study

From: Replacement of feline foamy virus bet by feline immunodeficiency virus vif yields replicative virus with novel vaccine candidate potential

Clones Viral stock namea Major mutation Effect on replication (CrFK)
pCF-7 [25] Wild-type FFVb
pCF7-BBtr FFV-BBtr Truncation at Bet amino acid 117 Fully susceptible towards feA3-mediated restriction in vitro
pCF7-BetMCS [50] FFV-BetMCS Insertion and replacement of Bet residues at amino acid 117 by insertion of a multiple cloning site Fully susceptible towards feA3-mediated restriction in vitro
pCF7-Vif-4 FFV-Vif-4 Engineered Bet-Vif fusion protein Partially susceptible towards feA3-mediated restriction in vitro
pCF7-Vif-39 FFV-Vif-39 Spontaneous frameshift Fully susceptible towards feA3-mediated restriction in vitro
pCF7-Vif W/*1 FFV-Vif W/*1b Trp to Stop mutation (TGG to TGA), unlinked vif gene Enhanced, compared to pCF7-Vif-4
pCF7-Vif W/*2 FFV-Vif W/*2 Trp to Stop mutation (TGGG to TAGA), unlinked vif gene Enhanced, compared to pCF7-Vif-4
pCF7-Vif W/*1 M+ Optimized upstream Met codon in pCF7-Vif W/*1 Similar to pCF7-Vif W/*1
pCF7-Vif W/*2 M+ Optimized upstream Met codon in pCF7-Vif W/*2 Similar to pCF7-Vif W/*2
pCF7-Vif W/*1 M/T Upstream Met codon mutated to Thr in pCF7-Vif W/*1 Similar to pCF7-Vif W/*1
pCF7-Vif W/*2 M/T Upstream Met codon mutated to Thr in pCF7-Vif W/*2 Similar to pCF7-Vif W/*2
  1. aFFV-Vif variants collectively referred to as “FFV Vif chimeras”
  2. bViral stocks used in domestic cat infection experiments