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Table 2 Cell lines tested for replication of ancML

From: Reconstruction of a replication-competent ancestral murine endogenous retrovirus-L

Cell Line

Organism

Lineage

GFP#

NEOa

L1.3b

ancMLc

DF-1

Gallus gallus

Aves

***

+++

++

−

CRFK

Felis catus

Carnivora

**

+++

+

−

CV-1

Cercopithecus aethiops

Primates

*

++

+

−

Vero

Cercopithecus aethiops

Primates

***

+++

−

+

HT1080

Homo sapiens

Primates

**

++

+

−

HOS

Homo sapiens

Primates

*

++

−

−

Huh7.5

Homo sapiens

Primates

*

++

−

−

HeLa

Homo sapiens

Primates

***

+++

++

−

pgsA745

Cricetulus griseus

Rodentia

**

++

+

++

CHO-K1

Cricetulus griseus

Rodentia

**

++

+

++

MusDunni

Mus dunni

Rodentia

***

+++

+

−

SC-1

Mus musculus

Rodentia

**

++

−

−

NIH3T3

Mus musculus

Rodentia

**

++

++

−

  1. All cell lines were transfected using lipofectamine 2000
  2. #A plasmid expressing GFP was utilized as a transfection control. Percentage of GFP positive cells: (−) 0%, (*) < 10%, (**) 10–50%, (***) > 50%
  3. aA pCR3.1 plasmid expressing a neo gene from a SV40 promoter was used as a control for G418 resistant colony formation
  4. bThe replication dependent LINE1 (L1.3) plasmid [41] was used as a control for retrotransposition and G418 resistance from the interrupted NEO reporter cassette
  5. cancML correspond to a pUC57 plasmid containing the cassette depicted in Fig. 3a
  6. Number of G418 resistant colonies: (−) None, (+) ≤ 10, (++) > 10, (+++) > 50