HIV-1 protease with leucine zipper fused at N-terminus exhibits enhanced linker amino acid-dependent activity

Retrovirology

Table 1 Primer sequences used for plasmid construction

Constructs	Forward primer (5′–3′)^a
DWzPR	5′ CTGTGGATCCTAACTTCCCTCAGGTAACGTTATGGCAA 3′-nt 2273
DWz/PR	5′ CGGGATCCTTCCTTTAACTTCCCTCAGGTCACGTTATGG 3′-nt 2270
DWz//PR	5′ CGGGATCCTACTGTATCCTTTAACTTCCCTCAGGTCACGTTATGG 3′-nt 2270
DWzPRV/P	5′ CGGGATCCTAACGTTCCTCAGATCACGTTATGG 3′-nt 2270
DWz/PRV/P	5′ CGGGATCCTTCCTTTAACGTTCCTCAGATCACGTTATGG 3′-nt 2270
DWz//PRV/P	5′ CGGGATCCTACTGTATCCTTTAACGTTCCTCAGATCACGTTATGG 3′-nt 2270
DWz/PSHL/PR	5′ CGGGATCCTCCCTCTCACCTCCCTCAGGTCACTCTTTGG 3′-nt 2270
DWz/PIDL/PR	5′ CGGGATCCTCCCATTGACCTCCCTCAGGTCACTCTTTGG 3′-nt 2270
DWz/PANF/PR	5′ CGGGATCCTCCCGCTAACTTCCCTCAGGTCACTCTTTGG 3′-nt 2270
p6*PSHL	nt 2221-5′ CCGATCGACAAGGAACTGTACCCTCTCACCTCCCTCAG 3′-nt 2258
p6*PIDL	nt 2221-5′ CCGATCGACAAGGAACTGTACCCATTGACCTCCCTCAG 3′-nt 2258
p6*PANF	nt 2221-5′ CCGATCGACAAGGAACTGTACCCGCTAACTTCC 3′-nt 2254

^a The numbers at the 3′ and/or 5′ ends denote HIV-1 proviral DNA nucleotide positions. Nucleotides corresponding to mutated amino acid residues are shown in boldface. Most of the primers contain BamHI sites (underlined) to facilitate cloning

ISSN: 1742-4690