Fig. 1

IFN treatment inhibits Vpx-mediated degradation of SAMHD1. THP1 cells were treated for 24 h with 1000 U/mL of IFNα, 1000 U/mL of IFNγ or left untreated. The indicated amount of VLPs-Vpx pseudotyped with VSV-G (as determined by RT activity) was added to cells for 16 h: cells were then collected and SAMHD1 degradation was measured by flow cytometry. a Representative flow cytometry pots. b Representative dose response experiment. THP1 were treated with the indicated amount of VLPs-Vpx. The percentage of cells in which SAMHD1 is degraded was determined as shown in a. c Combined data from three independent experiments, using a viral dose within linear range. *p < 0.05; **p < 0.01 (t test)