Fig. 4

Effects of NSC on dimerization of viral RNA. In vitro dimerization assays in the presence and absence of 10× molar ratio of NSC. RNA was heated to 95 °C and NSC in DMSO, or DMSO only, was added before snap-cooling on ice. RNA was then incubated for 1–2 h before native gel electrophoresis and densitometry analysis. a Dimerisation levels relative to the untreated RNA. Data are an average of five independent experiments, containing 1–3 samples of untreated/treated RNA each. Error bars show SD. b Representative samples from RNA renatured in the absence or presence of NSC, with (b) 2 h incubation at 37 °C or (c) 1 h incubation at 37 °C