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Fig. 3 | Retrovirology

Fig. 3

From: An RNA-binding compound that stabilizes the HIV-1 gRNA packaging signal structure and specifically blocks HIV-1 RNA encapsidation

Fig. 3

Viral gRNA levels analyzed by qRT-PCR, in the presence and absence of NSC. 293T cells were treated as in Fig. 2. 24 h post treatment, supernatants and cells were harvested, virions purified from 500 μL supernatant and RNA extracted. a gRNA levels in cells, analyzed by qRT-PCR. Each sample was first normalized against β-actin levels and then normalized against the average wild-type gRNA levels. Data shown are the average of 14 –18 samples taken from four independent experiments. Error bars represent SD. b Ratio of gRNA levels in purified virions from equivalent volumes of supernatant to gRNA levels inside cells. Intracellular levels were first normalized against β-actin; these values were then used to divide the virion RNA levels for each sample. Data were then normalized against the wild-type average. Data are shown as three independent experiments, each containing 4–9 replicates. c Ratio of gRNA levels in purified virions from equivalent volumes of supernatant to cytoplasmic gRNA. Data are representative of two independent experiments. d Ability of virions produced in the presence or absence of NSC to transduce cells, measured by luciferase assay. Error bars represent the SD. *p < 0.05; **p < 0.01 by Student’s t test. Data are representative of two independent experiments

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