Fig. 4

High mutation frequency in V1 and V2 loops of SIV Env variants in SIV-infected AGMs. a Mutation frequency in the protein sequence of plasma SIV Env variants isolated from SIVsab92018ivTF-infected AGMs and SIVmac251-infected RMs was quantified as the percent of Env variants showing a different amino acid compared to that of the challenge viral Env from the total number of variants. b Representative protein sequence alignments within V1V2 region of plasma SIV Env variants (depicted by each line) with mismatches highlighted relative to the respective wild-type challenge env (the top line). Hanging bars with SIVmac239 Env sequence number indicate relative positions of linear SIVmac V1 or SIVsab V2 IgG epitopes, and the two regions having high protein sequence variability in V1 and V2 loops. Purple circles indicate potential N-linked glycosylation sites (GS). Purple diamonds or blue empty diamonds indicate gained or lost potential N-linked GS in Env variants compared to corresponding challenge viral Env sequences, respectively