Fig. 2

Coverage of the 5′ region of the MuLV genomic RNA. Histograms show the number of 5′ read ends (with + 12 nt offset) mapped to viral RNA in RiboSeq HAR, RiboSeq CHX and RNASeq libraries generated from Rat2 cell infections, in reads per million (RPM). The light green rectangle represents the N-terminal extension of the Glyco-Gag-encoding sequence described in [16], while the darker green rectangle represents the standard gag coding sequence. The GUG initiation codon highlighted in green is in-frame with the gag N-terminal extension. Intervening AUC and AUG codons at which initiation was observed are shown in orange. Potential additional upstream initiation codons with coverage in the RiboSeq HAR library are shown in grey. Green, orange and blue bars correspond to reads mapping in phases 0, + 1 and + 2, respectively, relative to the gag ORF