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Fig. 6 | Retrovirology

Fig. 6

From: Inhibitors of the integrase–transportin-SR2 interaction block HIV nuclear import

Fig. 6

Representative hit compounds reduce nuclear import of HIV. Five hours after infection with IN-eGFP labeled virus in the presence of one of four representative hit compounds (MVG010, MVG044, MVG030 or MVG036), PF74 and DMSO or RAL as controls, HeLaP4 cells were fixed and analyzed by laser-scanning confocal microscopy. The ratio of nuclear/total amount of PICs was quantified (percentage nuclear PICs). a, b Representative slice of a stack of cells infected with eGFP-IN labeled virus in the presence of DMSO (a) or MVG044 (b). The nuclear lamina was immunostained with anti-lamin a/c (red). PICs are identified as green dots and nuclear PICs are highlighted by white arrows. c, d Presented are the cumulative distributions of the percentage of cells containing the indicated percentage nuclear PICs for the positive control PF74 (c) and four representative hit compounds (MVG010, MVG044, MVG030 or MVG036) or DMSO and Ral as controls (d). Two independent experiments, with distinct virus productions, are presented for each compound

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