Fig. 4

Raltegravir in vitro selections with patient-derived integrase recombinant viruses. Raltegravir concentration was doubled in each serial passage to a final concentration of 1024 nM of raltegravir in passage 10. All differences from the HXB2 reference sequence are given and mutations emerging during the raltegravir in vitro selections in the viral integrase coding region are indicated in red. The indicated mutations were detected by population sequencing of viral RNA in the culture supernatants from passage 10. Cultures 3 and 5 from Pt2 were discontinued due to failed virus propagation