Computational analysis of envelope glycoproteins from diverse geographical isolates of bovine leukemia virus identifies highly conserved peptide motifs

Table 1 Computational analyses indicate that conserved MEME motifs G–K of gp51 are under positive selection

MEME motift^a	dS^b	dN^c	Z-test	dN/dS ratio	Sites of positive selection^d	p values^e
A	0.03450	0.00629	< 0.0001	0.182	0	NA
B	0.03541	0.00253	< 0.0001	0.071	0	NA
C	0.08121	0.01129	< 0.0001	0.139	0	NA
D	0.09827	0.00000	< 0.0001	0.000	0	NA
E	0.09267	0.00133	< 0.0001	0.014	0	NA
F	0.09420	0.00000	< 0.0001	0.000	0	NA
G	0.11690	0.02867	< 0.0001	0.245	Lys⁷⁴, Arg⁷⁴	< 0.05
H	0.09437	0.04350	< 0.0001	0.461	Ala²⁹¹, Val²⁹¹, Thr²⁹¹	< 0.05
I	0.24183	0.01934	< 0.0001	0.080	Phe⁸², Ser⁸², Leu⁸²	< 0.05
J	0.14623	0.05945	< 0.0001	0.381	Val¹³³, Thr¹³³, Pro¹³³, Asp¹³³, Ala¹³³, Asp¹⁴¹, Asn¹⁴¹	< 0.05
K	0.10056	0.05619	< 0.050	0.559	Thr⁴⁸, Tyr⁴⁸, Val⁴⁸, Ile⁴⁸	< 0.05

^aMEME motifs A–F are highly conserved at the amino and nucleotide sequence levels, while MEME motifs G–K are conserved at the amino acid but not the nucleotide level
^bdS, rate of synonymous substitutions
^cdN, rate of non-synonymous substitutions
^dPositions of positive selection sites are the amino acid numbers beginning from the N-terminus of the precursor
^ep-values were calculated for the positive selection sites; NA, not applicable

ISSN: 1742-4690