Skip to main content
Fig. 5 | Retrovirology

Fig. 5

From: Moloney leukemia virus 10 (MOV10) inhibits the degradation of APOBEC3G through interference with the Vif-mediated ubiquitin–proteasome pathway

Fig. 5

MOV10 affects the assembly of Vif-CBF-β-Cullin 5-ElonginB-ElonginC Complex. ad The effect of MOV10 on the interaction between Vif and ElonginB (a), ElonginC (b), Cullin 5 (c), or CBF-β (d). 293T cells were transfected with pcDNA3.1-MOV10-FLAG (2 μg), pcDNA3.1-Vif-HA (1 μg), and 4 μg of ElonginB-FLAG (a) or ElonginC-FLAG (b) or pcDNA3.1-Cullin 5-FLAG (c) or pcDNA3.1-CBF-β-FLAG (d). After treated with MG132 (4 μM) for 16 h, cell lysates were immunoprecipitated with anti-HA agarose beads and analyzed by immunoblotting using anti-FLAG, anti-HA, and anti-GAPDH antibodies. In each transfection, empty vector pcDNA3.1 was used to normalize DNA amounts. Values in ad represent percentages of ElonginB-FLAG/ElonginC-FLAG/Cullin 5-FLAG/CBF-β-FLAG normalized against Vif-HA relative to control values. All the data is representative of at least three independent experiments

Back to article page