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Table 1 Biophysical antibody profiles

From: Systems serology: profiling vaccine induced humoral immunity against HIV

 
Fragment Fab Fc
Feature Antigen target Epitope target Antigen affinity Isotype Subclass Glycans FcR/C’ binding FcR affinity
Examples of measurements gp120 gp41
p24
Scaffolds
-V1V2
-SOSIP
-linear peptides
Dissociation constant
(k-off)
IgA
IgM
IgG
IgG1
IgG2
IgG3
IgG4
IgA1
IgA2
~30 glycan combinations
Eg. Fucose, bisecting GLNAc, Galactose, Sialic Acid
C1q
MBL
FcγRI
FcγRIIa
FcγRIIb
FcγRIIIa
FcγRIIIb
Equilibrium constants (KD)
Examples of Assays Multiplex
ELISAs
Multiplex
ELISAs
ICS
SPR
Chaotrope
Multiplex
ELISAs
Multiplex
ELISAs
Mass Spec
HPLC
CE
Multiplex
ELISA
Multiplex
SPR
References [104] [31, 40] [105] [104] [104] [53, 87, 106] [44, 87] [105, 107]