From: Systems serology: profiling vaccine induced humoral immunity against HIV
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Fragment | Fab | Fc | ||||||
Feature | Antigen target | Epitope target | Antigen affinity | Isotype | Subclass | Glycans | FcR/C’ binding | FcR affinity |
Examples of measurements | gp120 gp41 p24 | Scaffolds -V1V2 -SOSIP -linear peptides | Dissociation constant (k-off) | IgA IgM IgG | IgG1 IgG2 IgG3 IgG4 IgA1 IgA2 | ~30 glycan combinations Eg. Fucose, bisecting GLNAc, Galactose, Sialic Acid | C1q MBL FcγRI FcγRIIa FcγRIIb FcγRIIIa FcγRIIIb | Equilibrium constants (KD) |
Examples of Assays | Multiplex ELISAs | Multiplex ELISAs ICS | SPR Chaotrope | Multiplex ELISAs | Multiplex ELISAs | Mass Spec HPLC CE Multiplex | ELISA Multiplex | SPR |
References | [104] | [105] | [104] | [104] |