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Table 1 Biophysical antibody profiles

From: Systems serology: profiling vaccine induced humoral immunity against HIV

 

Fragment

Fab

Fc

Feature

Antigen target

Epitope target

Antigen affinity

Isotype

Subclass

Glycans

FcR/C’ binding

FcR affinity

Examples of measurements

gp120 gp41

p24

Scaffolds

-V1V2

-SOSIP

-linear peptides

Dissociation constant

(k-off)

IgA

IgM

IgG

IgG1

IgG2

IgG3

IgG4

IgA1

IgA2

~30 glycan combinations

Eg. Fucose, bisecting GLNAc, Galactose, Sialic Acid

C1q

MBL

FcγRI

FcγRIIa

FcγRIIb

FcγRIIIa

FcγRIIIb

Equilibrium constants (KD)

Examples of Assays

Multiplex

ELISAs

Multiplex

ELISAs

ICS

SPR

Chaotrope

Multiplex

ELISAs

Multiplex

ELISAs

Mass Spec

HPLC

CE

Multiplex

ELISA

Multiplex

SPR

References

[104]

[31, 40]

[105]

[104]

[104]

[53, 87, 106]

[44, 87]

[105, 107]