Fig. 2

In vitro Integration onto mononucleosomes. Either the 5′ biotinylated naked 601 DNA fragment or the native MNs (50 ng in DNA) were coupled to streptavidin beads and incubated with HIV-1 WT IN (400 nM) under integration conditions reported in the "Methods" section (a). After 0–2 h incubations the samples were deprotenized and washed after beads magnetization, then radioactivity was measured on both the pellet and supernatant. Quantification of the radioactivity remaining on beads after reaction performed with naked 601 DNA or MN and with or without IN is reported (b). The percentage of integrated product over time for each MN construct was reported in (c). Comparison of data obtained with IN alone and IN/LEDGF complex is reported in (d). All values are shown as the mean ± standard deviation (error bars) of three to four independent sets of experiments. The p values were calculated by Student’s t-test and are shown as *p < 0.05 to represent the probability of obtaining significant differences compared with the data obtained with the native MNs control