Fig. 4
AAV-mediated SaCas9/sgRNAs delivery suppresses HIV-1 infection in human primary CD4+ T cells. a A schematic diagram of AAV transfer vectors containing SaCas9 endonuclease and sgRNA. b T7E1 cleavage assay after 5 days post-transduction. c DNA sequences of CXCR4 of the AAV transduced CD4+ T cells. d Flow cytometry analysis of CXCR4 expression in AAV transduced CD4+ T cells. Assays were performed as in Fig. 1c. e CD4+ T cells counts at different times after transduction. CXCR4 disrupted cells continued to grow normally. f Flow cytometry analysis of apoptosis following AAV delivery SaCas9/sgRNA. g HIV-1 p24 was detected in the supernatants of the CD4+ T cells treated with AAV delivered SaCas9/sgRNA following HIV-1NL4-3 infection. For (d, e and f), one representative out of three independent experiments is shown. For (g), the graph represents 3 independent infection experiments and error bars represent SEM. Statistical analysis determined using unpaired t-test (***P < 0.001; **P < 0.01; *P < 0.05)