Fig. 3

Genome editing of CXCR4 in Jurkat T cells confers cell inhibition to HIV-1 infection by lentiCRISPR/SaCas9. a CXCR4 gene disruption analysis in Jurkat T cells by T7E1 cleavage assay. Assays were performed as in Fig. 1b. b Flow cytometry analysis of CXCR4 expression in lentivirus transduced Jurkat T cells. Assays were performed as in Fig. 1c. c DNA sequences of CXCR4 of the transduced Jurkat T cells. Assays were performed as in Fig. 1d. d HIV-1 p24 was detected in the Jurkat T cells treated with SaCas9/sgRNA. For (b), one representative out of three independent experiments is shown. For (d), the graph represents 3 independent infection experiments and error bars represent SEM. Statistical analysis determined using unpaired t test (***P < 0.001; **P < 0.01; *P < 0.05)