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Fig. 5 | Retrovirology

Fig. 5

From: Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication

Fig. 5

Codon modification of nucleotides 22-261 and 22-378 in gag decreases gRNA abundance and stability. HeLa cells were transfected with pHIV-1NL4-3, pHIV-1 CM22-261 or pHIV-1 CM22-378. a RNA was extracted from cell lysates at the 0 h timepoint and gRNA abundance was quantified by qRT-PCR. The bar charts show the average values of three independent experiments normalized to HIV-1NL4-3. Error bars represent standard deviation. b, c Actinomycin D was added to inhibit RNA polymerase II transcription and the abundance of MYC mRNA (b) or gRNA (c) was measured at 0, 1, 2, 4 and 6 h post-addition. Each value is relative to the 0 h timepoint and is an average of three independent experiments. Error bars represent standard deviation

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