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Fig. 3 | Retrovirology

Fig. 3

From: Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication

Fig. 3

Codon modification of nucleotides 22-261 in gag decreases gRNA abundance. HeLa cells were transfected with pHIV-1NL4-3, pHIV-1 CM22-261, pHIV-1 CM22-165 or pHIV-1 CM166-261. The bar charts show the average values of three independent experiments normalized to HIV-1NL4-3. Error bars represent standard deviation. a RNA was extracted from cell lysates and gRNA abundance was quantified by qRT-PCR. b RNA was extracted from the media and gRNA abundance was quantified by qRT-PCR. c Equivalent amounts of HIV-1 genomes were used to infect TZM-bl reporter cells to measure infectivity. The bar charts show the average values of three independent experiments normalized to the value obtained for HIV-1NL4-3. The average RLU for HIV-1NL4-3 is 1,146,196. The dashed line represents 1000 RLU, which is approximately the limit of the assay for reproducible differential results

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