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Fig. 2 | Retrovirology

Fig. 2

From: Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication

Fig. 2

Codon modification of nucleotides 22-261 in gag inhibits infectious virus production. HeLa cells were transfected with pHIV-1NL4-3, pHIV-1 CM22-261, pHIV-1 CM22-165 or pHIV-1 CM166-261. a Culture supernatants were used to infect TZM-bl reporter cells to measure viral infectivity. The bar charts show the average values of six independent experiments normalized to the value obtained for HIV-1NL4-3. The average relative light units (RLU) for HIV-1NL4-3 is 3,680,747. The dashed line represents 1000 RLU, which is approximately the limit of the assay for reproducible differential results. Error bars represent standard deviation. b, c Gag expression in the media (b) and cell lysate (c) was detected using quantitative immunoblotting. The bar charts show the average of four independent experiments normalized to HIV-1NL4-3. Error bars represent standard deviation

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