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Fig. 6 | Retrovirology

Fig. 6

From: The HIV-1 integrase-LEDGF allosteric inhibitor MUT-A: resistance profile, impairment of virus maturation and infectivity but without influence on RNA packaging or virus immunoreactivity

Fig. 6

CD4+ and CD8+ T-cell proliferation induced by autologous MDDCs exposed to MUT-A-inactivated HIV-1. a Proliferation in response to autologous MDDC exposed to wt HIV-1 (NL4-3 DMSO) or MUT-A inactivated HIV-1 or AT-2-inactivated HIV-1 in a 6-day co-culture was assessed in triplicates using the CFSE proliferation assay. MDDCs were pulsed with either 5 μg/mL HIV-1 (NL4-3 DMSO or MUT-A) or 1 μg/mL HIV-1 (NL4-3 AT-2) of HIV-1 gag CA-p24. A representative (out of three independent experiments) flow cytometry dot plots showing CFSE dilution in gated CD3+ CD4+ and CD3+ CD8+ T lymphocytes after in vitro stimulation with the above mentioned viruses or negative (medium) and positive SEA controls. b, c Quantitation of T CD4+ (B) and CD8+ (C) proliferation in response to autologous MDDC exposed to wt HIV virus (NL4-3 DMSO) or inactivated HIV virus (MUT-A or NL4-3 AT-2) in a 6-day co-culture was assessed in triplicates using the CFSE proliferation assay. In all viruses used, MDDCs were pulsed with 1 µg/mL of HIV gag CA-p24. Graph showing mean ± SD of percentages of CFSE low CD3+ CD4+ (b) and CD3+ CD8+ (c) T cells from three different co-cultures are represented

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