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Fig. 5 | Retrovirology

Fig. 5

From: The HIV-1 integrase-LEDGF allosteric inhibitor MUT-A: resistance profile, impairment of virus maturation and infectivity but without influence on RNA packaging or virus immunoreactivity

Fig. 5

T-cell proliferation induced by autologous MDDCs exposed to MUT-A-inactivated HIV-1 assessed by CFSE proliferation assay. Proliferation in response to autologous MDDCs exposed to wt HIV-1 (NL4-3 DMSO) or MUT-A or AT-2 inactivated HIV-1 in a 6-day co-culture was assessed in triplicates using the CFSE proliferation assay. MDDCs were pulsed with virus particles corresponding to 1 μg/mL of HIV-1 Gag CA-p24. A representative (out of 3 independent experiments) flow cytometry dot plots showing lymphocyte gating strategy by FSC/SSC and CD3+ positive staining, as well as dot plots showing CFSE dilution in gated CD3+ CD4+ T lymphocytes after in vitro stimulation with the above mentioned virus or negative (medium) and positive SEA controls

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