Fig. 4

Immunoreactivity of HIV-1 produced in the presence or absence of MUT-A. a Schematic of the antibody capture assay of native HIV-1 particles produced. The capacity of the different antibodies to capture native HIV-1 particles was assessed by ELISA. HIV-1 particles retained by the antibodies were lysed and quantified by CA-p24 detection by ELISA. b The different monoclonal and polyclonal antibodies used in virus-capture assays are listed together with their specific target and their ability to neutralize HIV-1. Nonspecific antibodies (polyclonal IgG HIV- and mAb Syn = Synagis) were used as negative controls. c Quantitation by CA-p24 ELISA of native HIV-1 particles produced in the presence (red bars) or absence (blue bars) of MUT-A that have been captured with the different antibodies used at three different concentrations