Fig. 4

Detection of T cell responses targeting the region containing the N323tc mutation. a Identification of potential cryptic epitopes in all ORFs at the N323tc mutation site. All potential ORFs on both DNA strands were compared between the T/F and mutant sequences at the mutation sites. Only a potential ORF at least 11 amino acids long is considered. Compared to the T/F sequences, the ORFs with amino acid substitutions (shown in red and blue in T/F sequences and the mutant sequences, respectively, at the mutation sites) are indicated in yellow. b T cell responses were detected with PBMCs from various days post screening and peptides containing potential cryptic epitopes at the N323tc site by the IFN-γ ELISpot assay. The dotted line indicates the threshold of a positive ELISpot response