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Fig. 3 | Retrovirology

Fig. 3

From: A strongly selected mutation in the HIV-1 genome is independent of T cell responses and neutralizing antibodies

Fig. 3

Detection of T cell responses targeting the region containing the K43R mutation. a Peptides containing the K43R mutation in the gag open reading frames (ORF) were used to detect T cell responses by the IFN-γ ELISpot assay. The dotted line indicates the threshold of a positive ELISpot response. b Identification of potential cryptic epitopes in all ORFs at the K43R mutation site. All potential ORFs on both DNA strands were compared between the T/F and mutant sequences at the mutation sites. Only a potential ORF at least 11 amino acids long is considered. Compared to the T/F sequences, the ORFs with amino acid substitutions (shown in red and blue in T/F sequences and the mutant sequences, respectively, at the mutation sites) are indicated in yellow. c T cell responses were detected with PBMCs from various days post screening and peptides containing potential cryptic epitopes at the K43R site by the IFN-γ ELISpot assay

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