Fig. 1

Human myeloid cells and HIV-1 infection. a Immunolabeling of cultured human fetal microglia (HFM) and bone marrow-derived macrophages (BMDMs) with antibodies to Iba-1 (HFM: orange, BMDMs: orange) and MHC Class II (HFM: yellow, BMDMs: yellow) expression. b Relative CD68, CD163, CD4 and CCR5 transcript expression levels in HFM and BMDMs were quantified by RT-PCR. c HFM and BMDMs were infected at a multiplicity of infection (MOI) of 0.1 with HIV-1 YU-2 (derived by transfection in 293 T-cells) and supernatant was collected after every 4 days for HIV-1 p24 quantification by ELISA