Fig. 1

SDS-PAGE analysis of purified IgGs and antibody fragments targeting CD4i epitopes. Purified IgG of each mAb against the CD4i epitopes (a), and IgG, Fab and scFv of three mAbs, 916B2, 4E9C and 25C4b (b), were analyzed by SDS-PAGE. IgG was purified from the culture supernatant of transformed B cells or transfected 293A cells with a Protein A column. Fabs were purified from the culture supernatants of transfected 293A cells with a Ni column. ScFvs from E. coli strain Rosetta 2 (DE3) transformed cells were purified with a Ni column and refolded. M, size marker