Fig. 6

FASN inhibition disrupts a late step in the HIV-1 replication cycle. a TZM-bl cells were transfected with pNL4-3 provirus plasmid for 48 h in the presence or absence of 10 µM Fasnall or 10 µM C75 or DMSO (0.01%). Intracellular expression of HIV-1 proteins was monitored by HIVIG-western blot, and β-actin was used as loading control. b Supernatant associated virion production was monitored using a p24 ELISA. c 48 h post-transfection, cell culture supernatants were removed and incubated with fresh TZM-bl (indicator) cells for an additional 48 h. Values expressed as the mean ± standard deviation, and are representative of three independent experiments. p-values were generated with student’s t test. Molecular weight markers (kD) indicated in right margin