Fig. 5
From: Cellular fatty acid synthase is required for late stages of HIV-1 replication
FASN knockdown reduces HIV-1 particle production without affecting intracellular Gag production. a FASN immunoblot confirms reduction in endogenous FASN levels in TZM-bl cells. b Extracellular (left) and intracellular (right) p24 levels following siRNA-mediated FASN knockdown in TZM-bl cells infected with HIV-1 for 48 h. Bars represent mean p24 ± SD, quantified using a commercial ELISA, n = 3, p-values generated with student’s t test. c anti-Gag western blot of lysates from HIV-infected cells with or without FASN knockdown. Molecular weight markers (kD) indicated in right margin. Poly = polymer (transfection) control, NT = nontargeting siRNA (siRNA control), FASN = FASN-targeted siRNAs, NVP = 0.4 µM nevirapine