Fig. 2
From: Cellular fatty acid synthase is required for late stages of HIV-1 replication
HIV-1 infection of TZM-bl cells does not alter FASN mRNA levels but it does increase FASN protein and fatty acid levels. a FASN mRNA levels, normalized to 18S rRNA levels, following infection with HIV-1NL43 for the indicated number of hours post infection (h.p.i). Normalized mRNA levels at 4 h post infection are not significantly different than normalized mRNA levels at time 0, p = 0.2, Student’s t test. Data are representative of two independent experiments. b Western blot analysis of FASN and actin protein levels in TZM-bl cell lysates [left], THP-1 lysates following pcTAT transfection or HIV-1 infection [middle], or SupT1 lysates [right] with or without HIV-1 infection. ImageJ software was used to calculate relative FASN expression normalized to actin expression. c Fatty acid quantification by gas chromatography was performed on extracts of TZM-bl cells following 48 h of infection with 0, 20, or 40 ng p24/mL of HIV-1. The data presented are mean values (± SD) from four independent experiments. * indicates p < 0.05 (Student’s t test). h.p.i = hours post infection