Fig. 1
From: Cellular fatty acid synthase is required for late stages of HIV-1 replication
Purine-binding proteins regulated by HIV-1 infection. TZM-bl cells were HIV-infected and the TZM-bl purinome was captured 48-h post-infection. Proteins that remained bound to the resin after a high ionic wash were competed off the resin with 25 mM ATP, resolved by one-dimensional SDS-PAGE, visualized with silver stain, and identified with MALDI-TOF sequencing as the following: (1) Ubiquitin carboxyl terminal hydrolase (Q9Y4E8), (2) ATP-dependent RNA helicase DHX8 (Q14562), (3) FASN (P49327), (4) HSP90-beta (P08238), (5) GDP-L-fucose synthetase (Q13630), (6) L-lactate dehydrogenase (P07195) and pyridoxial kinase (O00764), (7) Argininosuccinate synthase (P00966), (8) Nucleoside diphosphate kinase-A (P15531) and, (9) Nucleoside diphosphate kinase–B (P22392)