Fig. 3

Virus production in DNA-transfected 293T cells. The wt and 3 mutant DNA constructs were transfected in 293T cells and virus production was measured after 2 days by CA-p24 ELISA (a) or an RT activity assay (b) on the culture supernatant. Average values obtained in three independent transfection experiments are shown with error bars indicating standard deviations. Each supernatant sample was analyzed multiple times for CA-p24 (threefold) and RT (twofold). Reduced viral gene expression for Max compared to wt and Low reaches borderline significance (student T test)