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Fig. 4 | Retrovirology

Fig. 4

From: Granulocytic myeloid-derived suppressor cells suppress virus-specific CD8+ T cell responses during acute Friend retrovirus infection

Fig. 4

Depletion of MDSCs. B6 mice were infected i.v. with 20,000 SFFU of FV, and/or treated with 5FU or αLy6G. CD8+ numbers were measured using flow cytometry and viral loads were estimated for spleen cells at 14 days post infection. Experimental design of the MDSC depletion by administration of 5FU or αLy6G (a). b Representative dot plots of MDSC during FV infection after administration of 5FU, αLy6G or untreated. c The numbers of CD43+ CD8+ T cells, the numbers of FV-DbgagL class I tetramers positives CD8+CD43+, and the numbers of proliferating Ki67+CD43+CD8+ T cells per 1 × 106 live cells in the spleen are displayed. d The numbers of CD43+ CD8+ T cells expressing GzmB per 1 × 106 live cells in the spleen are displayed. e The numbers of viral loads (infectious centers per 1 × 106 cells) in the spleen are displayed. At least five mice per group from three independent experiments were analyzed. Bars represent the mean with SD. For statistical analysis a Dunn’s test with the Benjamini–Hochberg correction for multiple testing was performed (*<0.05; **<0.005)

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