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Fig. 3 | Retrovirology

Fig. 3

From: Granulocytic myeloid-derived suppressor cells suppress virus-specific CD8+ T cell responses during acute Friend retrovirus infection

Fig. 3

Mechanisms of MDSC mediated suppression. B6 mice were infected i.v. with 20,000 SFFU of FV or left un-infected and the expression of Arg1 and NOS2 in the MDSCs was measured using flow cytometry for spleen cells at day 14 post infection. Frequencies of NOS2, Arg1, PD-L1, and CD39 expressing Ly6Ghigh Ly6Clow cells in the spleen are displayed (a). At least five mice per group from three independent experiments were analyzed. Bars represent the mean with SD. For statistical analysis an unpaired t test was performed (*<0.05; ***<0.0005). b CD8+ T cells isolated from DbGagL TCR transgenic mice were incubated with dendritic cells loaded with MHC class I-restricted FV-specific CD8+ T cell epitope peptide and co-incubated with gMDSCs in the ratio of 1 CD8+ to 10 gMDSCs with or without the addition of L-NMMA or nor-NOHA. Representative histograms and percentages of proliferating CD8+ T cell proliferation after co-incubation with or without gMDSCs in the presence or absence of L-NMMA/nor-NOHA are shown. At least three independent experiments were analyzed. Bars represent the mean with SD. c CD8+ T cells isolated from DbGagL TCR transgenic mice were incubated with dendritic cells loaded with MHC class I-restricted FV-specific CD8+ T cell epitope peptide and co-incubated with gMDSCs isolated from CD39 KO mice or from PD-L1 KO mice. Representative histograms and percentages of proliferating CD8+ T cells after co-incubation with or without gMDSCs isolated from B6, CD39 KO and PD-L1 KO FV infected mice are shown. At least three independent experiments were analyzed. The bars represent the mean with SD. For statistical analysis a Dunn’s test with the Benjamini–Hochberg correction for multiple testing was performed (*<0.05; **<0.005)

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