Fig. 8

Effect of FACT-mediated chromatin remodeling chemical promotion on early steps of HIV-1 in LEDGF/p75 knock-out context and on PFV infectivity. Similar experiments than described in Fig. 7 were performed in TZM and TZM knock out for LEDGF/p75 (a, b) or in HEK293T cells transduced either with HIV-1 or PFV vectors (c). The effect on the early steps of lentiviral vector replication was evaluated by quantifying GFP-positive cells. All values are shown as the mean ± standard deviation (error bars) of at least three independent sets of experiments. The p-values were calculated by Student’s t-test and are shown as *p < 0.05 and **p < 0.005 to represent the probability of obtaining significant differences compared with untreated conditions