Fig. 3

Effect of LEDGF/p75 on FACT-mediated stimulation of in vitro HIV-1 integration. Concerted integration assays were performed as done in Fig. 2 but using IN•LEDGF complex, naked or chromatinized acceptor DNA and increasing concentrations of FACT (a). Results are reported as the percentage relative to activity detected on naked DNA without FACT and pre-normalized data obtained in the control experiments using naked DNA and without FACT are reported as the percentage of integrated substrate. Results obtained with full length LEDGF/p75 or the LEDGF 326–471 IBD-GST domains are reported in (b). All values are shown as the mean ± standard deviation (error bars) of at least three independent sets of experiments. The p-values were calculated by Student’s t-test and are shown as *p < 0.05 and **p < 0.005 to represent the probability of obtaining significant differences compared with control experiments performed with PN DNA without FACT