Fig. 4

CypA-capsid interaction mediates LV restriction independent from CypA expression. a iPSC were transduced with LV at an MOI of 100 in the presence of 10 µM CSA. CSA was added at indicated time points prior to or during transduction. The percentage of EGFP and SSEA1 double positive cells from 3 independently produced viral supernatants were assessed by flow cytometry. Repeated measures one-way ANOVA with Dunnett post hoc test was used for statistical analyses. *** p ≤ 0.001; ** p = 0.003. b Transduction of iPSC with LV, harboring wt or mutated HIV-1 capsids. The percentages of EGFP and SSEA1 double positive cells were quantified by flow cytometry (wt n = 9, wt + CSA n = 9; G89V n = 3; P90A n = 3; H2.1 n = 3; A88T n = 5; independently produced viral supernatants). One-way ANOVA with Dunnett post hoc test was used for statistical analyses. wt versus wt + CSA, *** p ≤ 0.001; wt versus G89V, * p = 0.014; wt versus P90A, ns p = 0.066; wt versus H2.1, *** p ≤ 0.001; wt versus A88T, ** p = 0.002; wt versus N74D, ns p = 0.9996. c Western blot analysis showing cellular CypA protein levels in iPSC, C57BL/6 p14f/f adult fibroblasts and CF1-Mefs. The percentage of the CypA/Erk2 ratio is depicted for each cell type relative to iPSC