Fig. 4
From: Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity
Coexpression of Gag chimeras containing HERV-K CA-NTD reduces HIV-1 release efficiency. a pCRVI/HeHeMHe-1, HeMHeHe-1, MHeMM-1, MMHeM-1, HeHeMHe-2, HeMHeHe-2, MHeMM-2 and MMHeM-2 encode CA-domain chimeras between HERV-K Gag and MLV Gag. HERV-K MHR and MLV MHR are shown in green and blue, respectively. This same color coding is used to indicate constructs containing the different MHRs in the subsequent panels in Figs. 4, 8, and 9. b, d Cell and viral lysates from cotransfected cells were subjected to SDS-PAGE and analyzed by immunoblotting with HIV-Ig or anti-Flag antibody. c, e HeLa cells were transfected with pNL4-3 and indicated plasmids. The amount of p24 was measured as described in Fig. 3c. P values, compared with HERV-K Gag, were determined using a Student’s t test. *P < 0.01; **P < 0.001; ***P < 0.0001; n.s. not significant