Fig. 3
From: Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity
Coexpression of Gag chimeras containing HERV-K CA reduces HIV-1 release efficiency. a pCRVI/HeHeM, HeMHe, MHeHe, MMHe, MHeM and HeMM encode chimeras between HERV-K Gag and MLV Gag. All chimeric Gag constructs are tagged with the Flag epitope. b Cell and viral lysates from cotransfected cells were subjected to SDS-PAGE and analyzed by immunoblotting with HIV-Ig or anti-Flag antibody. c HeLa cells were cotransfected with the HIV-1 molecular clone pNL4-3 and indicated plasmids. The amount of p24 in the virus pellet was measured by ELISA. An empty vector pCRVI was used as a control. pCRVI/HERV-K Gag-Flag and pCRVI/MLV Gag-Flag express Flag-tagged HERV-K Gag and MLV Gag, respectively. Data from three independent experiments are shown as means ± standard deviations