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Fig. 2 | Retrovirology

Fig. 2

From: Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity

Fig. 2

Coassembly with HERV-K Gag changes the size of particles containing HIV-1 p24 Gag. HeLa cells were separately transfected or cotransfected with the HIV-1 molecular clone pNL4-3 and indicated plasmids. For analysis of particles containing single Gag (gray square), the supernatants from separately transfected HeLa cells were collected and pooled. For coassembled particles (black square), the supernatant of cotransfected HeLa cells was used. These viruses were fractionated in rate-zonal gradient analysis. The amounts of HIV-1 Gag were measured by ELISA (a), and the amounts of HERV-K Gag were measured by immunoblotting using anti-Flag antibody (b). Representative data from five independent experiments are shown

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