Fig. 4

APOBEC3/Rfv3-dependent NAb response requires type I IFN signaling. Mice were infected with FV/LDV at two different inoculum doses: a, b 10,000 SFFU and c, d 2000 SFFU. a, c Plasma samples at 28 dpi were heat-inactivated and the reciprocal plasma dilution that conferred 50% neutralization was computed. Log₄-transformed data are shown and used for statistical analyses. b, d FV-specific IgG2b/c titers were determined by endpoint-titration ELISA for mice infected with 10⁴ SFFU of FV/LDV. Native FV virions were coated into ELISA plates and twofold dilutions of plasma were added. IgG2b/c antibodies were detected using a combination of anti-IgG2b and anti-IgG2c conjugates. In all panels, each dot corresponds to a mouse and lines correspond to mean values. The total number of mice analyzed was combined from 2 independent experiments. Data were analyzed using 2-tailed unpaired Student’s t test; ns not significant (p > 0.05)