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Fig. 6 | Retrovirology

Fig. 6

From: Identification of a tripartite interaction between the N-terminus of HIV-1 Vif and CBFβ that is critical for Vif function

Fig. 6

Identification of CBFβ mutants that are defective in Vif binding and restoring infectivity in the presence of A3G. a Representative western blots of 293T cells co-transfected with CBFβ siRNA or control siRNA, along with Flag-CBFβ, WT Vif, and 0.34 or 0.67 μg of Flag-A3G. The blots show Flag-A3G, Flag-CBFβ, endogenous CBFβ, WT Vif, and tubulin (loading control). b Infectivity of viruses produced in experiments shown in (a) as determined by measuring luciferase activity in TZM-bl cells. The bar graphs represent relative infectivity compared to no A3G samples (set to 100%). All data represent mean ± SDs from two independent transfection experiments; virus infectivity for each virus stock was an average of three infections. Statistical significance was determined using t test; *P < 0.05

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