Fig. 2

Effect of selected double-alanine mutations in Vif on A3G degradation and HIV-1 infectivity. a Stick diagram of amino acids at the Vif–CBFβ interface. ⁵WQVMVIW¹¹ amino acids of Vif and amino acids 55–68 of CBFβ, and the interaction between W5 of Vif with I55 and F68 of CBFβ are shown. b Representative immunoblots of Flag-A3G, Vif, and tubulin (loading control). Degradation of Flag-A3G was determined by co-transfection of either 0.34 or 0.67 μg of Flag-A3G expression plasmid. c Quantitation of Vif-induced A3G degradation relative to the no Vif control (which was set to 100%) is shown. Bar graphs represent mean and standard deviations of two or more independent transfection experiments and statistical significance was determined using t test; *P < 0.05. d Infectivity of viruses in the presence of different Vif mutants. Data (mean ± SDs from two or more independent experiments done in triplicates) are plotted relative to viruses produced in the presence of WT Vif (set to 100%). Statistical significance was determined using t test; *P < 0.05