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Fig. 7 | Retrovirology

Fig. 7

From: Conserved residues within the HIV-1 Vpu transmembrane-proximal hinge region modulate BST2 binding and antagonism

Fig. 7

E28A/L33A mutation affects the ability of Vpu to target CD4. a, b E28A/L33A is attenuated for CD4 degradation. HEK293T cells were co-transfected with a proviral construct encoding WT Vpu or the indicated Vpu mutant proviruses and a CD4 expressor, and probed for the steady state levels of CD4. a Shown is a representative Western blot. b A summary of the densitometric quantifications of the steady-state CD4 levels from independent experiments, together with the SD (n = 7). Statistical analysis was performed using a two-way ANOVA, Tukey’s multiple comparison test. c E28A/L33A is efficient at recruiting β-TrCP. HEK293T cells were co-transfected with the indicated proviral constructs and a myc-tagged β-TrCP2 expressor. A Co-IP assay was performed using anti-myc Abs to analyze β-TrCP complexes for the presence of Vpu. d E28A/L33A is attenuated for CD4 binding. HEK293T cells were co-transfected as in (a), and a Co-IP assay performed using anti-CD4 Abs to pull down Vpu. Shown is a representative Western blot indicating the amount of Vpu in the lysate and pulled down fractions in each case. The asterisk denotes an Ab-related band

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