Fig. 2

E28/L33 residues have no intrinsic activity on Vpu cellular distribution in the absence of BST2. a Intracellular localization of Vpu mutants in HeLa cells depleted of BST2 (SH-BST2, treated with shRNA against BST2) or not (NT-BST2, treated with non-targeting shRNA). Transfected cells were co-stained with anti-TGN46 (red, for TGN) and anti-Vpu (green) Abs as well as with DAPI (grey, for nucleus). Shown are representative confocal microscopy pictures for each of the Vpu mutants. The white bars represent a distance of 10 µm. b Quantification of the co-staining of anti-Vpu and anti-TGN46 Abs obtained from at least 50 distinct transfected cells per mutant. Shown are PCC values from each mutant. The horizontal lines represent the mean PCC. Statistical analyses were performed using Mann–Whitney test