DNA hypomethylation and aberrant expression of the human endogenous retrovirus ERVWE1/syncytin-1 in seminomas

Retrovirology

Table 1 Percentage of 5-mCpG and 5-hmCpG on the ERVWE1 promoter

ERVWE1 promoter	Total 5-mCpG + 5-hmCpG^a Bisulfite sequencing %	5-mCpG^b Oxidative bisulfite sequencing %	5-hmC^c %
T3 (seminoma)	6	7	−1
T3-matched control	52	49	3
T4 (seminoma)	29	27	2
T4-matched control	93	83	10
T5 (seminoma)	44	48	−4
T5-matched control	86	87	−1
T6 (seminoma)	6	8	−2
T59 (lymphoma)	88	91	−3
Placenta	26	23	3
BeWo	0	1	−1
TCam-2	66	66	0

^aThe percentage of total 5-mCpG + 5-hmCpG modification of the ERVWE1 promoter was obtained after the conventional bisulfite sequencing
^bThe percentage of 5-mCpG modification of the ERVWE1 promoter was obtained after the oxidative modification of the bisulfite sequencing
^cThe percentage of 5-hmCpG modification of the ERVWE1 promoter was obtained by subtracting the percentage of 5-mCpG from the percentage of total modified CpG. Such subtraction produced negative values in several cases. The P value calculated using the Mann–Whitney two-tailed test was >0.05, i.e. non-significant in all samples

ISSN: 1742-4690