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Table 1 Percentage of 5-mCpG and 5-hmCpG on the ERVWE1 promoter

From: DNA hypomethylation and aberrant expression of the human endogenous retrovirus ERVWE1/syncytin-1 in seminomas

ERVWE1 promoter Total 5-mCpG + 5-hmCpGa
Bisulfite sequencing
%
5-mCpGb
Oxidative bisulfite sequencing
%
5-hmCc
%
T3 (seminoma) 6 7 −1
T3-matched control 52 49 3
T4 (seminoma) 29 27 2
T4-matched control 93 83 10
T5 (seminoma) 44 48 −4
T5-matched control 86 87 −1
T6 (seminoma) 6 8 −2
T59 (lymphoma) 88 91 −3
Placenta 26 23 3
BeWo 0 1 −1
TCam-2 66 66 0
  1. aThe percentage of total 5-mCpG + 5-hmCpG modification of the ERVWE1 promoter was obtained after the conventional bisulfite sequencing
  2. bThe percentage of 5-mCpG modification of the ERVWE1 promoter was obtained after the oxidative modification of the bisulfite sequencing
  3. cThe percentage of 5-hmCpG modification of the ERVWE1 promoter was obtained by subtracting the percentage of 5-mCpG from the percentage of total modified CpG. Such subtraction produced negative values in several cases. The P value calculated using the Mann–Whitney two-tailed test was >0.05, i.e. non-significant in all samples