Fig. 5

Inhibition of autophagy promotes PFV replication, and activation of autophagy reduces its replication. a Western blotting was used to analyze the expression of the viral protein Tas in PFV-infected cells in the absence or presence of 3-MA or Rapa. BHK-21 cells were pre-treated with 3-MA (10 mM) or Rapa (400 nM) for 4 h, followed by infection with PFV at an MOI of 0.5. The intracellular virus yields were determined by measuring viral protein at 24 hpi. b Western blotting was used to analyze the expression of Atg5 in cells that down-regulated Atg5 in response to specific shRNAs. BHK-21 cells were transfected with Atg5-specific shRNA1 or shRNA2 for 48 h. The cell samples were harvested and lysed, and cell extracts were measured by immunoblotting with anti-Atg5 antibody. c BHK-21 cells were transfected with Atg5-specific shRNA1 or shRNA2 for 48 h and then infected with PFV (MOI = 0.5) for another 24 h to analyze the expression of the viral protein Tas in PFV-infected cells that down-regulated Atg5 in response to specific shRNAs. Significance was analyzed with a two-tailed Student’s t test. **P < 0.01, ***P < 0.001